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RIKEN Plant Science Center

RIKEN Genomic Sciences Center

MAEDA(Micro Array Expression DAta search)

Our policy to Microarray

  Microarrays are powerful tools for the systematic analysis of expression profiles of large numbers of genes, including stress-inducible gene expression, tissue-specific gene expression, and changes in the expression profiles of mutants and transgenic plants. We have used the full-length cDNAs for the microarray analysis of expression profiles of Arabidopsis genes under various stress and hormone treatment conditions, and in various mutants and transgenic plants.
  After the publication of our papers on expression profiling, expression data on this work will be available at this site. Our microarray database should be useful for analysing the expression profiles of plant genes in response to environmental stress and hormone treatments.

Note

1.We used a full-length cDNA microarray containing ca. 7000 Arabidopsis full-length cDNAs, including drought-inducible genes, responsive to dehydration (rd) and early responsive to dehydration (erd), as positive controls, the PCR-amplified fragment from lambda control template DNA fragment (Takara) as an external control, and the mouse nicotinic acetylcholine receptor epsilon-subunit (nAChRE) gene and the mouse glucocorticoid receptor homolog gene, which have no substantial homology to any sequences in the Arabidopsis database, to assess for nonspecific hybridization as negative controls (Seki et al. 2002). Only the normalization method using the PCR-amplified fragment from lambda control template DNA fragment (Takara) as an external control gene is now available at this site.

References

1. Seki,M, Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y. and Shinozaki, K. (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J. 31: 279-292.
2. Seki, M., Ishida, J., Narusaka, M., Fujita, M., Nanjo, T., Umezawa, T., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y. and Shinozaki, K. (2002) Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray. Functional and Integrative Genomics 2:282-291.

The microarray experimental data(s) which can be referred to.

1.Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold
and high-salinity stresses using a full-length cDNA microarray.
(Seki M. et. al. Plant J 2002 Aug;31(3):279-292) [PubMed]
2.Identification of Arabidopsis genes regulated by high light-stress using cDNA microarray.
(Kimura M. et. al. Photochem Photobiol. 2003 Feb;77(2):226-33) [PubMed]
3.Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA
treatments using a full-length cDNA microarray.
(Seki M. et. al. Plant J 2002 Nov;2(6):282-91) [PubMed]
4.Monitoring expression profiles of Arabidopsis gene expression during rehydration
process after dehydration using ca 7000 full-length cDNA microarray.
(Oono Y. et. al. Plant J 2003 Jun;34(6):868-87) [PubMed]

-Acquire the gene names from expression data.
-Acquire the expression data from gene names.

-Blast for genes on microarray.