K19728

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K19728

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K19728

Determination of introduced cDNA(s)

  • J013095H11 - Rice Full-length cDNAs - Original: J013095H11

    full reading [+] show sequence

    Nucleotide sequence: J013095H11 - full reading 
    GCTCTCTCTC CCGTGTCTCT CGCCGCGGCG TCCTCCGGCC ACCACCCCGC 
    GCCGCGCCGC CGTCTACGGA TCCCCGCTCC TCGCTCGAGC GAGCACGAAA 
    GCCCTAGCGC GCCGCCCCCT GCACCGGGTG GGTTCCCCAG GTTGTTGAGC 
    TAGAATGGGA GTGGCAGGGC ACAATGATCC TTTGATAGGT GAAACGACAT 
    GTGGATCTTT GCTGCAGCAA CTTCAGTTGA TATGGGATGA GGTTGGTGAA 
    AGCGATGAAG ACCGTGACAA GATGTTACTT CAGCTAGAAC AAGAATGCTT 
    GGATGTTTAC AGGAGAAAGG TTGATCAGGC ATCTAATTCC AGGGCACGCC 
    TCCTTCAACA GCTTGCTAAC GCCAAGTCTG AGCTTTCTAG ACTCCTTTGT 
    GCACTAGGCG AGCTGTCTAT TTCTGGCATT CCTGACAAGA CAACCGGTAC 
    TATCAAGGAG CAACTAGAAG CAATATCACC TTTCTTGGAG AAACTCTGCA 
    GGGAAAAAGA TAAGAGGGTT AGAGAGTTTG CTGGTGTACA ACTTCAAATT 
    CAAACTATAC GTGGTGAAAT CGCTGGGAGT CTGCAAGTTG GTGACCACAT 
    GGAAACACCC CGTGTTAATG AGGATGATCT TTCAACGAAG AAATTAAATG 
    AATTTCTTTC TGAATTGCAA GCTCTACAAA AGGAAAAGAG CAATAGACTG 
    CACAAGATTC TTGACTTCGT GAGTTCAGTG CATGATCTCT GTTCTGTCCT 
    TGGGATGGAT TTCCTGAGCA CTGTTACTGA AGTTCATCCT AGTCTCAATG 
    ACTCGGTTGG TGCCGAGTTC AAGAGCATTA GTGACGCAAC ATTATCCAAG 
    CTGTCTAAGA TGGTAATTCA ACTTAAAGAA GAGAAATCAA AGAGACTTGA 
    AAGGATTCAA GCTCTAGCTT CCCAGCTAAC TGATCTTTGG AACCTAATGG 
    ACACCTCTGC TGATGAGCGA CAACTTTTTG ACCATGTCAC ATGCAACATT 
    TCCTCAACAT TGGATGAAGT AACAGCACCA GGAGCTCTTG ATATTGATTT 
    GATCGAACAG GCTGAACTTG AAGTTGAAAG GCTGGATCAG CTCAAAGCTA 
    GTAGGATGAA GGATATTGCG TTCAAAAGGC AGACTGAACT TGAAGATATA 
    TATGCCCAGG CTCACATTAC GATAGATACC AGTGCTGCAA GAGATAGAAT 
    ACTGACTGTT ATTGATTCTA GTATCTTCGA GCCTTCGGAA CTACTGGCTG 
    ATATGGAGAA CCAGATACTT AAAGCAAAAG AAGAGGCACT AAGCAGAAAG 
    GACATTCTGG AGAAGGTTGA AAGGTGGATG TCAGCATGCG AAGAAGAGAG 
    CTGGCTTGAA GACTATAGCC AGGACGACAA CAGATACAGT GCAACCAGAG 
    GTGCACATCT GAACCTCAAG CGGGCAGAAA AGGCTCGTCT TTTGGTCAGT 
    AAAATTCCAG TCATTGTTGA TACACTGATG GCAAAGACCC GGGCATGGGA 
    ACAAGAGCAT GGTATGCCGT TCAGCTATGA CGGTGTTCAT CTTCTCGCAA 
    TGCTGGATGA GTACAAAGTA CTAAGGCAGC AGAAGGAAGA AGAGAAGCGA 
    CGAATGAGGG ACCAGAAGAA GATAAACGAC CAGCTAGCTG CAGAGCAGGA 
    GAAGCTCTTC GGCTCGAAAC CGAGCCCAGC CCGGCCCCAA TCCTCAAGGA 
    AGGCGCCTGG CCCGCGGGCC AACGGTGGCG CAGTCAATGG CACACCGAAC 
    CGGAGGCTCT CGGCCCACCA GAACGGCGGC GGCAGGTCGG TGAGCCGGGA 
    CGGGCGGAGG GACAGTGGCC GGCCGGCTGC TCCGGTGAAC TACGTCGCCA 
    TCTGCAAGGA AGAGTCCAGC AACAACAACC CGGCGGCGTC CTCCCCCTGA 
    CGATCATCCT CGCCATGATT GACCGTACAT TTCTTGGTGC CAGTGGTAGA 
    GAGATACTCC ATATCTGTGC AGTAGCAGGA AAAAAAGAAA AACTATAGCA 
    AGATACTAGT AGGACAAGTG ATTCGAATGA TAGTAGCAGT ATTTTGGCTG 
    TATTTGAATC GATTTGTTGC TGTCCATTTG CTGGTAGTGG ATCGAATGGA 
    TCGAGTGGGT GCAGCCGTGC AGGTGCATCC GCCATGGAAG CTGAGCTCAT 
    GTCGTCGTGT CCCGTTGG

    Gene models with high sequence identity

    1. AT5G55230.1 [+] show detail - microtubule-associated proteins 65-1
      E-value: 0; Score: 702.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Microtubule-associated protein, MAP65/ASE1-type 2.5e-72
    HMMPanther Microtubule-associated protein, MAP65/ASE1-type 1.2e-130
    Coil coiled-coil 0
    Coil coiled-coil 0
    Coil coiled-coil 0
    Coil coiled-coil 0

    See the detailed result >

    AT5G55230.1

    Model type
    Protein coding
    Short Description
    microtubule-associated proteins 65-1
    Curator Summary
    Binds and bundles microtubules. Plays a role in stabilizing anti-parallel microtubules in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. The timing with which the MAP65-1 was targeted to the spindle appears to be regulated by a phosphorylation sensitive switch. Enhances microtubule polymerization, promotes nucleation and stabilizes microtubules against cold treatment and dilution.
    Computational Description
    microtubule-associated proteins 65-1 (MAP65-1); FUNCTIONS IN: microtubule binding; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Microtubule-associated protein, MAP65/ASE1-type (InterPro:IPR007145); BEST Arabidopsis thaliana protein match is: microtubule-associated protein 65-2 (TAIR:AT4G26760.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR