K18824

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) abnormal (PATO:0000460) (Rosette leaf::Others)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Shape::zigzag)
T2
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) abnormal (PATO:0000460) (Rosette leaf::Others)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Shape::zigzag)

Invisible phenotypes

Silique::Shape::narrow

Original: K18824

Determination of introduced cDNA(s)

J013038G02 - Rice Full-length cDNAs - Original: J013038G02

full reading [+] show sequence

GATATCTAGA AAAACAGAAA AAGAATATAA GGGGGAAGAC TTGGGCCGCC 
GCTAACCCTA ACCCCTGGCG ACGGCGGCCG ATCGTCGTCG ACGAAGTCAG 
GCGCGGAGGC TGCGACCGGA TTAGCCGGTC GCCCCTGGAT TTGGAGCTCC 
CGTGTCCGTG TGGGGGCAAT GCTAGCGCGG GCGTTTGGCC TCGTGGGCAA 
AACATGGAAG CGAACGCCGA CTATGTCCGT CGAGGCTTCC GCCTCGCCGC 
GCCGCCGCTC CCAGATGCCA CAGCAGCAGC TTTGTCATCT CCACGGCAGC 
ACGCCGTCAC AACGGCAAAA GTTCCCCGAA GAACAGCTCC CGGGAGGAGC 
TCCGCCAATG CTGGACTATA TCTCCGAACA GAACTTAAGC ACCGTAGTAA 
TGGCAACATG GAGAATTCAG CTGTTGCTGC TGTCGACTCT AATGGATAAC 
CTTGCTCGCT TCTGAACTTC AATATTGAAC TAAGCTTTGT AAGGTTGGCA 
TGCTAAATTA GTTGTTTACA TTCAGGGATA CTGTTGAGGG GAGTTTTGAC 
CTGAGGTTTG CAGCACTGAA GAGCATTGGG GAGGATCGGG TCAACGATCG 
TGAGCTTAAG TTACTGCTAA AGAAGAAACA TGCCCCCATT TGTTATGTTT 
GGTGTGATCC ATGCCCCTGG ATGCACATTT CTCAGGTACA ATATTGAGAT 
GTGGAAAGCA ACTGGCATGG CTCTTGATAA AGTAGATCTA GTCTGGTTGT 
CAGATGAAAT ACGTCAACAT GGTGATGAGT ACTGGCCAAT TGTCATGGAT 
ATTGCAAGGA CAAACAGTGT GCGTAGAATA AAAAGTACAT GCAGGTCCTC 
TTATTACTGT TTACCTAGCA GATTTTGTGG GAGTAGAAAT TCTTATGCGA 
TTGGAAAATT AAATGTTGAT GAGATTTTTT ACATTTGCTT GCAGTGTGCT 
AGCATATTAT TTCAGAAGGC AGACATATGG CTGTTGGGAA TGGAACAGCA 
TGATGCCAAC TTATTAGCTA GACAATATTA TAAGCATTTC AAAAAGAAGA 
ATAAGGCAAT TGCTGTTTTG GACAATATGC TTCCTAATTT ATTACAATAC 
CCTCAAATGG AGAACAGGAG GCATCCAGCT TGGGCTATCT TCATGGAAGA 
TGATAAGGAG GATATATGTT TTAAAATGGA AAAAGCTTTC TGCCCTCCAA 
AACTAGCAGA AGGCAATCCA TGTTTGGAGT ACATAAAATA TATCATCTTG 
CCTTGGTTTG GAATGTTTGA GGTGGTCCAG AAGAAAGGGA ATGGTGGCAA 
AAAGACTTTT CTGAGCATGG AGGAACTTAC AGCTGATTAT GAAAGTGGCG 
CTCTGCATCC TGCTGATGTG AAGCTGGCCC TTGAAAAGTC ATTGAACGAG 
ATATTGCAGC CTGTTCGTGA TCACTTTGGA AGGAATGGTG AAGCAAAGGA 
TCTAGTGGAA GCTATCGGGG AATGCTACGG TGCTGACTGA TATTACATGT 
TCACTCTTGG ACTTAGGAGT AGTGTTCTTA TCTTTGGCCA TGTAATGAAC 
AAATCAATCC CTATCTATGA GCAGTCTTAG AGGTTCCTTT TGTGCCACAA 
GGTCATCAGT TCAGTGCTTG CTCTTTCCTC GTGGGTGTGT TAGAAGGGTG 
TCTTTTTTTT TCCTCATCAC AACCTTGTTT CTTGTATTTT TTACCTGCTA 
TATGAATATG AAACCTCCCG CTGTCTCGTG CGGTTAGTTC CC

Gene models with high sequence identity

AT2G33840.1 [+] show detail - Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial
E-value: 8e-65; Score: 245.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Aminoacyl-tRNA synthetase, class Ib	5e-21
HMMPIR	Tyrosine tRNA ligase, archaeal/eukaryotic	7.7e-114
Gene3D	Rossmann-like alpha/beta/alpha sandwich fold	2.9e-13
Gene3D	no description	1e-32
superfamily	Nucleotidylyl transferase	5.6e-41
HMMPanther	Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic	4.9e-39
HMMPanther	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHION	4.9e-39

See the detailed result >

AT2G33840.1

Model type: Protein coding
Short Description: Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial
Computational Description: Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial; FUNCTIONS IN: tyrosine-tRNA ligase activity, nucleotide binding, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: translation, tRNA aminoacylation for protein translation, tyrosyl-tRNA aminoacylation; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Tyrosine tRNA ligase, archaeal/eukaryotic (InterPro:IPR016485), Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic (InterPro:IPR015624), Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (InterPro:IPR002307), Aminoacyl-tRNA synthetase, class Ib (InterPro:IPR002305); BEST Arabidopsis thaliana protein match is: Nucleotidylyl transferase superfamily protein (TAIR:AT1G28350.1); Has 5443 Blast hits to 5419 proteins in 1791 species: Archae - 389; Bacteria - 3121; Metazoa - 323; Fungi - 286; Plants - 124; Viruses - 5; Other Eukaryotes - 1195 (source: NCBI BLink).
Link: InterPro Scan - TAIR