K12945

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	increased width (PATO:0000600) (Rosette leaf::Shape::wide) present in fewer numbers in organism (PATO:0001997) (Rosette leaf::Number::few)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)
T2
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	epinastic (PATO:0000945) (Cauline leaf::Shape::epinastic) abnormal (PATO:0000460) (Cauline leaf::Others)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) epinastic (PATO:0000945) (Rosette leaf::Shape::epinastic) dark green (PATO:0001249) (Rosette leaf::Color::dark)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K12945

Determination of introduced cDNA(s)

J023134L09 - Rice Full-length cDNAs - Original: J023134L09

full reading [+] show sequence

GAAAGGAACT TCTTTTCTGA AGACTGATTA AAAAAACATG ACCTTTGAAT 
CAAGCTTGTG GCCTCTCAGA TAACGAACTT TTCCTGCCAC CTAGTTATGA 
GTATAATATG GCTTACATTT ACACAGGTGA AAATGAAAAT GAGAAAAGAA 
AAGGCACAAT AAGAAACAAC GGCTGATGAT TTATTATGTT CTGCCCTTCT 
TTGTTGTGAC AAAGTATCTT GATCACAAGG ACGGAATTGG CAGGGTAGCT 
GGCAGTTGCA GTAGCATTAA CTGCCAACTG TGTGCTGCTA ATCTTATTTG 
ATCCAAATTA TATGGTTATT AATCAAATCC CATAGCCCCA TAATCCCCAA 
CCATGCCCGA AGAAGCTGCT GGGATGGCGT CAGTCTTGCG GAGGCTCTAC 
CTCTCTGTCT ACAACTGGAT TGTCTTCATC GGATGGGTTC AGGTGTCGTG 
GTCCATGATA TTGGCATTGT TGGAGAAGAG ATATGAGGCC GTCTACGCTG 
CTGTAGAGCA GCATCTGCTG TTCGCGCAGA CTGCTGCCAT CATGGAGATT 
CTTCATTCCA TTGTAGGGTT GGTGAGGTCT CCAGTGTCTT CTACTCTTCC 
ACAAATTACT GCAAGGTTGT TCATGATCTG GGGCATCTTG CGGAGCTTCC 
CTGAGATACA CACTCATATT TTTGTTACCT CCTTGCTCAT AAGCTGGTGC 
ATCACTGAGG TTACCAGATA TTCTTTCAAT GGCATGAAGG AGTCATTTGG 
ATTTACACCT TCCTGGCTCT TATGGCTTAG GTACAGCACC TTCATAGTAT 
GCTTTCCTAT TGGTATGGTT AGTGAGGTTG GCCTAGTCTA CATTGTTGTT 
CCTTTCATGA AGGCATCTGA GAAATACTGC CTTAGGATGC CCAACAAATG 
GAATTTCTCC ATCAAATACT TCTATGCCTC TGTCTTTTTC ATGGCTTTGT 
ATGCCCCAGT ATACCCGCAC TTGTTCCACT ATTTGATCGT CCAGCGGAAG 
AAGGCCTTGG CAAAATCAAA AACCACATAA CTACTCAAGA GCTATCTAAT 
CTGGTTTCTG CTCGCAGCTA CAGCTCAGGT TGCTACCACT TCTTGAACAA 
AGAAGTAAAA ATGTATTAGA ATGGAAATGC CGAACTACAC AGACTATCTT 
TTCCCTTGGT AAATAAAAAT GGACATGAGT ACTATTTTCA TGATTTGTTA 
ATGGTGTGTT GTACCAAACT TTGGCTTTTC CATATGTAAA TAAAATTGCA 
CTCCATATCG ATGCC

Gene models with high sequence identity

AT5G10480.1 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
E-value: 8e-72; Score: 268.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Protein-tyrosine phosphatase-like, PTPLA	3e-53
HMMPanther	PHOSPHATASE RELATED PROTEIN PTPLA	2.5e-59
HMMPanther	Protein-tyrosine phosphatase-like, PTPLA	2.5e-59

See the detailed result >

AT5G10480.1

Model type: Protein coding
Short Description: Protein-tyrosine phosphatase-like, PTPLA
Curator Summary: Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
Computational Description: PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR