K11948

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K11948

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • increased width (PATO:0000600)
    (Cauline leaf::Shape::wide)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
T2  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
seed (PO:0009010)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K11948

Determination of introduced cDNA(s)

  • J033120E09 - Rice Full-length cDNAs - Original: J033120E09

    full reading [+] show sequence

    Nucleotide sequence: J033120E09 - full reading
    GATACTCTCT CTCCTCCTCC TCCTCCACTC CGGCGACTCC TCCGCCGCAT 
    CCCGAGCAAG CAAGCCGCGC CGCCGACGAC CTAGCTCCCC GATCGCGGCC
    ATGTCGAGGC GCGGCGACTG GGTCTACGAG AACAACGGCG GGACGTGCGT
    GGCCATCGCC GGCGCGGACT ACTGCGTGGT GGCCGCCGAT ACCCGCCTTT
    CCGTCGGATA CAACATCCTC ACGCGCGACC ACTCCAAGAT CTGCGAGCTA
    GCGGACAAAT GCGCACTAGC ATCTTCCGGC TTCCAAGGTG ATATCAAAGC
    TCTGCACAAG AACTTGGCTG CCAGAGAATT GCTGTACCAG CACCAGCACA
    ATAAAAGGAT GAGCTGCCCT GCCATGGCAC AACTGCTCTC CAACACTCTG
    TACTACAAGC GATTCTTCCC ATATTATGCT TTCAATGTAC TTGGTGGGCT
    TGATAGTGAA GGGAAAGGAT GTGTTTTCAC CTATGATGCA GTTGGGTCCT
    ATGAGAGGAC TGGCTACAGT GCACAGGGAA CAGGATCTGC TTTGATCATG
    CCAGTGCTGG ACAACCAACT AAAATCACCT AGTCCACTAT TACTCCCTGC
    AAGAGATGCC GTCACACCTT TGTCGGAGAC AGAGGCGGTC GATTTAGTGA
    AGGATGTGTT TGCATCGGCA ACTGAGAGGG ACATCTACAC TGGTGATAAG
    CTGGAGATAG TTGTCATCAA CAAAGCTGGG ACGAAGCGAG AGTACATCGA
    CCTGAGGAAG GACTAGATCG ACGATTATGT TCTTCAGTTA CCGCAAATTT
    GCATCAACAA ATTTGCTTCG AACATTCTCC CTGATACGCC AGGGAATCAT
    GCCCTGAACT TGATAGAAAC GCCTGTGCTT TCTTGCTTCT ATTCTGTACC
    AGGATTCTCC CAACCTGCTT TGTTGAACTA ATCGCTGCCT TGATGCAAAT
    GAGACCGAAC ATATTTATTG TCAGAAATAT CACAATGGTC GATATGTTGA
    ACTGTGCTTG TGTTTGCTCG ATATTCGATA TGTTTATTG

    Gene models with high sequence identity

    1. AT3G60820.3 [+] show detail - N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein
      E-value: 8e-96; Score: 347.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily N-terminal nucleophile aminohydrolases (Ntn h 2.9e-55
    HMMPanther PROTEASOME SUBUNIT BETA TYPE 1 1.4e-113
    HMMPanther PROTEASOME SUBUNIT ALPHA/BETA 1.4e-113
    Gene3D no description 7.3e-56
    PatternScan Proteasome, beta-type subunit, conserved site 0
    HMMPfam Proteasome, subunit alpha/beta 4.5e-34

    See the detailed result >

    AT3G60820.3

    Model type
    Protein coding
    Short Description
    N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein
    Curator Summary
    Encodes 20S proteasome beta subunit PBF1 (PBF1).
    Computational Description
    PBF1; FUNCTIONS IN: peptidase activity, endopeptidase activity, threonine-type endopeptidase activity; INVOLVED IN: defense response to fungus, incompatible interaction, ubiquitin-dependent protein catabolic process; LOCATED IN: proteasome core complex, proteasome complex; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Proteasome, beta-type subunit, conserved site (InterPro:IPR016050), Proteasome, subunit alpha/beta (InterPro:IPR001353); BEST Arabidopsis thaliana protein match is: proteasome beta subunit C1 (TAIR:AT1G21720.1).
    Link
    InterPro Scan - TAIR