GT7150

CSHL Arabidopsis genetrap mutant lines

Original: GT7150

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal
  • small or tiny plants
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
whole plant (PO:0000003)
  • decreased height (PATO:0000569)

Original: GT7150

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    Not mapped

    Flanking Genome Sequence: GT7150 - 3-prime edge 
    TATACCTTCA CAAAACATAA AACT

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1224465 [Map]; Strand: w
      • AT5G04360.1 - [+] show detail - limit dextrinase
        Distance: 0; Region: intron11 (CDS)
    Flanking Genome Sequence: GT7150 - 3-prime edge 
    TTAAGAGCCT GCCAAAAGAA GCTACATAAA GAGCCAACTC GAAATAGCAT 
    GAGGCTTTAT CATGCAGTCA TCAACACATT GTATGAATCA CTACAAATTT 
    TAGCTTTCAA TGAGCCTATT AGTATTGCAA ATAACCAGGA CCAGTATACA 
    CTTACTATTT TCAATATCCA AACATGGGAA AAATGCAAAC CTGAACCATT 
    TTTCTAAATT CAATTATGCG GCATGGACCA GTCGGATCAC TAGCCTAGCT 
    TCCTTTTGGG ACTCCCCAGA GCACAGGATT ATACCTTCAC AAAACATAAA 
    ACTTGTGATG AAGGTGATTG ATTCATTATG AAGAATGAAG TAACTTGTGT 
    AAAACAAACT ACCCCCAGTT ATAGCCATCA TCATTCTGGA TTTCTGTAAT 
    ACG

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1224603 [Map]; Strand: w
      • AT5G04360.1 - [+] show detail - limit dextrinase
        Distance: 0; Region: exon12 (CDS)
    Flanking Genome Sequence: GT7150 - 5-prime edge 
    ATGTCGTCTT AGATGTTGTT TACAACCACT TGCATGCAAG TGGGCCACAT 
    GACAAAGAAT CTGTTCTTGA TAAGGTGTGC ACCTAACTAA GATTTGATTA 
    ATTTTCAGTT TGATAACTAA CTGTCTAGCC TGAATTCTCA GCTATCTTCC 
    ACGAAAAAAT GTATTTGCTT AGATAGTTCC AGGTTACTAT TTGAGAAGGA 
    ACAGTGATGG TTTTATTGAA AACAGTACAT GCGTAAACAA CACTGCTAGC 
    GAGCACTATA TGGTTGATCG TCTCATACGG GATGATCTAT TAAATTGGGT 
    TGTTAACTAT AAGGTACATG CTTGTAATCT CTTCTGATTA TTTTCCATCT 
    TGCTACTTCT TCCCTCCTTG AAAAATATAT TAGCATTTTT CTCATGTATC 
    TTTCAACCTT TGATCGACTG GCCATGACAA TGTTCATTAT ATCCTGCAAA 
    GTATATTTTT TGC

AT5G04360.1

Model type
Protein coding
Short Description
limit dextrinase
Curator Summary
Encodes an enzyme thought to be involved in the hydrolysis of the α-1,6 linkages during starch degradation in seed endosperm. However, a knockout mutant of Arabidopsis lacking limit dextrinase has normal rates of starch degradation in the leaf at night, indicating that more than one isoamylases might be involved in this process.
Computational Description
limit dextrinase (LDA); FUNCTIONS IN: limit dextrinase activity, pullulanase activity, alpha-amylase activity; INVOLVED IN: starch biosynthetic process, carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Alpha-1,6-glucosidases, pullulanase-type (InterPro:IPR011839), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 6260 Blast hits to 6253 proteins in 1613 species: Archae - 73; Bacteria - 5462; Metazoa - 45; Fungi - 1; Plants - 262; Viruses - 0; Other Eukaryotes - 417 (source: NCBI BLink).
Link
InterPro Scan - TAIR