F24226

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	abnormal (PATO:0000460) (Cauline leaf::Others)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) increased size (PATO:0000586) (Rosette leaf::Shape::large) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	root (PO:0009005)	present in greater numbers in organism (PATO:0000470) (Root::Number::many)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick) abnormal (PATO:0000460) (Stem::Others) present in fewer numbers in organism (PATO:0001997) (Stem::Number::few)
T2
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small) dark green (PATO:0001249) (Cauline leaf::Color::dark)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) dark green (PATO:0001249) (Rosette leaf::Color::dark) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick) abnormal (PATO:0000460) (Stem::Others) present in fewer numbers in organism (PATO:0001997) (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F24226

Determination of introduced cDNA(s)

RAFL05-02-G21 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-G21 - Resource: pda00842

full reading [+] show sequence

AAAAACTCAC CGACGCGATC CGATCTCATC TCTCTCATTT CGAAACCATG 
GTTGAGCCGG CGAATACTGT TGGTCTTCCG GTGAACCCGA CTCCGTTGCT 
GAAAGATGAG CTCGATATCG TGATTCCGAC TATCAGAAAC CTCGATTTCC 
TCGAGATGTG GAGGCCTTTT CTTCAGCCTT ACCATCTGAT CATCGTCCAG 
GACGGAGATC CATCGAAGAA GATCCATGTC CCTGAAGGTT ACGACTACGA 
GCTCTACAAC AGGAACGACA TTAACCGAAT CCTCGGACCT AAGGCTTCTT 
GTATCTCGTT TAAGGATTCT GCTTGTCGAT GCTTTGGGTA CATGGTGTCT 
AAGAAGAAGT ATATCTTCAC CATTGATGAC GATTGCTTCG TTGCCAAGGA 
TCCATCAGGC AAAGCAGTGA ACGCTCTTGA GCAACACATC AAGAACCTTC 
TCTGCCCATC GTCTCCCTTT TTCTTCAACA CCTTGTATGA TCCTTACCGT 
GAAGGTGCTG ATTTCGTCCG TGGATACCCT TTCAGTCTCC GTGAAGGTGT 
TTCCACTGCT GTTTCCCATG GTCTTTGGCT CAACATCCCT GACTACGATG 
CCCCGACCCA ACTCGTGAAG CCTAAGGAGA GGAACACCAG GTATGTGGAT 
GCTGTCATGA CCATCCCAAA GGGAACACTT TTCCCAATGT GTGGTATGAA 
CTTGGCTTTT GACCGTGATT TGATTGGCCC GGCTATGTAC TTTGGTCTCA 
TGGGTGATGG TCAGCCTATT GGTCGTTACG ACGATATGTG GGCTGGTTGG 
TGCATCAAGG TGATCTGTGA CCACTTGAGC TTGGGAGTGA AGACCGGTTT 
ACCGTATATC TACCACAGCA AAGCGAGCAA CCCTTTTGTT AACCTGAAGA 
AGGAATACAA GGGAATCTTC TGGCAGGAGG AGATCATTCC GTTCTTCCAG 
AACGCAAAGC TATCGAAAGA AGCAGTAACT GTTCAGCAAT GCTACATTGA 
GCTCTCAAAG ATGGTCAAGG AGAAGTTGAG CTCCTTAGAC CCGTACTTTG 
ACAAGCTTGC AGATGCCATG GTTACATGGA TTGAAGCTTG GGATGAGCTT 
AACCCACCAG CAGCCAGTGG CAAAGCTTGA GAGCAGTATG AGCCAAAAAG 
AAAAAGCCAC CAAAGTTTTG GTTATTTTTA GCTCAAATTA TCGTTACTTT 
TAAATTTCTG ATTTTACGAA CCTTTCTTGC TTTTTTTACA CATTTGAGTA 
GTTTTCATCA TCAGTACTTT CTCATTGTCC GGTTATGGTT TTTGCATTTG 
GTTTAAATAT CACCGGTTTA TTTATAAACA GTGGTGGATT AGTAGTACTA 
TTTTCTGAGT TTTTTTCTTT GTTTCATTAA TAAAAAGGCC TTTTCATAGG 
TGTTTGCAAC T

Gene models with high sequence identity

AT5G15650.1 [+] show detail - reversibly glycosylated polypeptide 2
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPIR	Alpha-1,4-glucan-protein synthase, UDP-forming	1.3e-271
HMMPfam	Alpha-1,4-glucan-protein synthase, UDP-forming	2.1e-186

See the detailed result >

AT5G15650.1

Model type: Protein coding
Short Description: reversibly glycosylated polypeptide 2
Curator Summary: Reversibly Glycosylated Polypeptide-2. Constitutive expression in tobacco impairs plant development and virus spread.
Computational Description: reversibly glycosylated polypeptide 2 (RGP2); FUNCTIONS IN: transferase activity, transferring hexosyl groups, glycogenin glucosyltransferase activity; INVOLVED IN: response to cadmium ion, response to salt stress; LOCATED IN: cytosolic ribosome, cell wall, membrane; EXPRESSED IN: male gametophyte, guard cell, root, cultured cell, leaf; EXPRESSED DURING: seedling growth, seed development stages; CONTAINS InterPro DOMAIN/s: Alpha-1,4-glucan-protein synthase, UDP-forming (InterPro:IPR004901); BEST Arabidopsis thaliana protein match is: reversibly glycosylated polypeptide 1 (TAIR:AT3G02230.1); Has 259 Blast hits to 255 proteins in 45 species: Archae - 24; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 223; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink).
Link: InterPro Scan - TAIR