F20740

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F20740

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
T2  
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • increased length (PATO:0000573)
    (Silique::Shape::long)
  • decreased thickness (PATO:0000592)
    (Silique::Shape::thin)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F20740

Determination of introduced cDNA(s)

  • RAFL05-05-L13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-05-L13 - Resource: pda01057

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-05-L13 - full reading 
    CTGGGAGGAG AGAGGCCGCC GGAGGAGAGA GAAAAAGAGT TGGGGGATTG 
    CTTTTTGATT TCTCCTTCTC CATCTTCGAC CTCGAGAGAA GCTAAAAATG 
    GCAGAAGAAG CGGATACGAA CATCGAGATC TGGAAGATTA AGAAACTAAT 
    CAAGGGACTT GAATCAGCTA GAGGTAATGG AACGAGTATG ATCTCTCTTA 
    TTATGCCTCC TCGTGATCAA GTATCTCGTG TCACAAAGAT GCTTGGTGAT 
    GAATATGGAA CTGCTTCAAA CATTAAGAGT CGAGTCAATC GTCAATCTGT 
    TTTGTCTGCC ATTACTTCTG CTCAACAAAG GTTGAAGCTT TACAACAAGG 
    TTCCTACTAA TGGTCTTGTT CTCTATACCG GCACCATTGT TAATGATGAC 
    GGTAAAGAGA AGAAGGTGAC GTTTGATTTC GAGCCCTTTA GGCCGATTAA 
    TGCTTCCTTG TACCTTTGTG ACAACAAGTT CCATACTGAG GCATTGAACG 
    AGCTTCTTGA GTCTGATGAC AAGTTTGGTT TCATTGTTAT GGATGGTAAT 
    GGGACACTGT TTGGAACTTT GAGTGGGAAT ACTAGAGAGG TTCTTCATAA 
    GTTTACTGTT GATCTCCCGA AGAAGCACGG AAGAGGAGGA CAGTCTGCAC 
    TTCGTTTTGC CCGTCTTAGG ATGGAGAAGA GACACAATTA TGTTAGGAAA 
    ACCGCTGAGC TTGCTACACA GTTTTACATC AATCCAGCTA CGAGTCAGCC 
    TAATGTCTCT GGTCTGATCC TTGCTGGTTC AGCTGATTTC AAAACTGAGC 
    TCAGTCAATC AGAGTTGTTT GATCCTCGTC TTCAAGCTAA GATATTGAAT 
    GTTGTTGATG TTTCTTACGG AGGTGAAAAC GGTTTCAACC AAGCGATTGA 
    GCTGTCTGCT GAGATTCTAT CCAATGTGAA GTTCATACAG GAGAAGAAAC 
    TGATTGGGAA GTACTTTGAG GAGATAAGTC AAGATACCGG GAAATACGTT 
    TTCGGTGTGG AGGATACTCT AAAGGCTCTA GAAATGGGTG CTATCGAAAC 
    CCTTATCGTG TGGGAGAATC TGGACATAAA CAGGTATGAG CTAAAGAACA 
    GTACGACAGG AGAAATGGTG GTGAAACACT TTGGGAAAGA TCAAGAGTCT 
    GATACAAGCA ACTTCCATGA TTCAGAGACT AACGCGGAGC TAGAAGTACA 
    GGAGAAGATG CCTCTACTCG AATGGTTTGC AAATGAGTAC AAACGTTTCG 
    GCTGCACTCT TGAGTTTGTG ACTAACAAAT CCCAAGAAGG GTCACAGTTT 
    TGCAGAGGTT TTGGTGGGAT TGGTGGTATG TTACGTTACC AGCTTGACAT 
    GAGGACATTT GATGAGTTAT CAGATACTGA AGTTTACGAA GATTCTGATT 
    AAAGTGGGAA AACTTTTGGT GATGGTTTCA GATCGATACA GTGAGGGAAA 
    ATGTGGCATA AAGATGGTCT TTTATCAACA AAGAAGAGAG ATTTAAGTTT 
    CTTGTTTTCT ATAGAACATT AAAAAGCTTG ACTCTTTCTT TCTGTTAGAC 
    AATTTGTGTT GTAATTTTTG AGGATTTTAA ACATCTTTTT GTTGTTAAAA 
    AAAAAAAAAA AAAAAAACAA AAAAAAAAAA AAAA

    Gene models with high sequence identity

    1. AT1G12920.1 [+] show detail - eukaryotic release factor 1-2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily N-terminal domain of eukaryotic peptide chain 3.6e-56
    superfamily L30e-like 2e-50
    superfamily Translational machinery components 5e-48
    HMMPfam eRF1 domain 2 5.5e-42
    HMMPfam eRF1 domain 3 4.9e-38
    HMMPfam eRF1 domain 1 4.2e-28
    Gene3D no description 1.5e-42
    Gene3D no description 4.4e-39
    Gene3D no description 2.2e-59
    HMMPanther EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUN 0
    HMMPanther Peptide chain release factor eRF1/aRF1 0
    HMMTigr eRF: peptide chain release factor eRF/aRF, 1.9e-211
    HMMTigr Peptide chain release factor eRF1/aRF1 4.3e-154

    See the detailed result >

    AT1G12920.1

    Model type
    Protein coding
    Short Description
    eukaryotic release factor 1-2
    Curator Summary
    Encodes a eukaryotic release factor one homolog.
    Computational Description
    eukaryotic release factor 1-2 (ERF1-2); FUNCTIONS IN: translation release factor activity; INVOLVED IN: translational termination; LOCATED IN: translation release factor complex, plasma membrane; EXPRESSED IN: leaf; CONTAINS InterPro DOMAIN/s: eRF1 domain 2 (InterPro:IPR005141), eRF1 domain 3 (InterPro:IPR005142), eRF1 domain 1 (InterPro:IPR005140), Peptide chain release factor eRF1/aRF1 (InterPro:IPR004403); BEST Arabidopsis thaliana protein match is: eukaryotic release factor 1-3 (TAIR:AT3G26618.1); Has 1141 Blast hits to 1136 proteins in 380 species: Archae - 310; Bacteria - 0; Metazoa - 243; Fungi - 154; Plants - 143; Viruses - 3; Other Eukaryotes - 288 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR