F18010

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	rosette leaf (PO:0000014)	present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Shape::zigzag)
T2
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) abnormal (PATO:0000460) (Rosette leaf::Others) present in fewer numbers in organism (PATO:0001997) (Rosette leaf::Number::few) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F18010

Determination of introduced cDNA(s)

RAFL04-17-E23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-E23 - Resource: pda00633

full reading [+] show sequence

AGTCATGTTT CCATAGACAT AATCTCTCAC CATTGTTTTT CTCTGCAAAA 
CTAAAGAAAC AAAAAAAGAA AAATCGGAGA AACCAAGAAA AAAGAAATGG 
GAAGTGTAGA AGCAGGAGAA AAGAAGGCTT TAGGATGGGC TGCAAGAGAT 
CCTTCTGGTG TTCTCTCTCC TTACTCCTAC ACTTTAAGGA GCACAGGAGC 
AGATGATGTG TATATAAAGG TTATATGCTG TGGAATATGC CATACTGATA 
TTCACCAAAT TAAAAATGAT CTTGGAATGT CTAATTATCC TATGGTTCCT 
GGGCACGAGG TAGTAGGTGA GGTGTTGGAA GTGGGATCAG ATGTGAGCAA 
GTTCACCGTA GGAGATGTAG TCGGAGTCGG CGTGGTCGTT GGATGCTGCG 
GTAGCTGCAA ACCATGCAGC TCAGAACTTG AGCAGTATTG TAACAAGAGA 
ATCTGGAGTT ACAACGATGT CTACACCGAT GGAAAACCTA CTCAAGGTGG 
CTTCGCCGAC ACCATGATCG TCAATCAAAA GTTTGTGGTG AAGATTCCAG 
AAGGAATGGC GGTGGAGCAG GCTGCACCGC TACTCTGCGC GGGAGTAACG 
GTTTATAGTC CGTTGAGCCA CTTCGGTCTA ATGGCGAGTG GGCTTAAAGG 
AGGGATTCTA GGGTTAGGTG GAGTAGGGCA CATGGGTGTG AAGATAGCTA 
AAGCAATGGG ACACCATGTG ACTGTGATAA GCTCAT

Gene models with high sequence identity

AT3G19450.1 [+] show detail - GroES-like zinc-binding alcohol dehydrogenase family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
superfamily	GroES-like	6.3e-49
superfamily	NAD(P)-binding domain	4.3e-09
Gene3D	no description	3.8e-55
PatternScan	Alcohol dehydrogenase, zinc-containing, conserved site	0
HMMPanther	ZINC-TYPE ALCOHOL DEHYDROGENASE-RELATED	2.6e-65
HMMPanther	Alcohol dehydrogenase superfamily, zinc-containing	2.6e-65
HMMPfam	Alcohol dehydrogenase GroES-like	2.6e-27

See the detailed result >

AT3G19450.1

Model type: Protein coding
Short Description: GroES-like zinc-binding alcohol dehydrogenase family protein
Curator Summary: Encodes a catalytically active cinnamyl alcohol dehydrogenase which uses p-coumaryl aldehyde as a preferred substrate. It can also use caffeyl, coniferyl and d-hydroxyconiferyl aldehydes as substrates.
Computational Description: ATCAD4; CONTAINS InterPro DOMAIN/s: GroES-like (InterPro:IPR011032), Alcohol dehydrogenase GroES-like (InterPro:IPR013154), Alcohol dehydrogenase, zinc-containing, conserved site (InterPro:IPR002328), Alcohol dehydrogenase, C-terminal (InterPro:IPR013149), Alcohol dehydrogenase superfamily, zinc-containing (InterPro:IPR002085); BEST Arabidopsis thaliana protein match is: cinnamyl alcohol dehydrogenase 5 (TAIR:AT4G34230.1); Has 40533 Blast hits to 40509 proteins in 3018 species: Archae - 756; Bacteria - 27152; Metazoa - 1406; Fungi - 3053; Plants - 2651; Viruses - 3; Other Eukaryotes - 5512 (source: NCBI BLink).
Link: InterPro Scan - TAIR