F16950

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F16950

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
whole plant flowering stage (PO:0007016)
  • premature (PATO:0000694)
    (Adult plant::Flowering::early)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16950

Determination of introduced cDNA(s)

  • RAFL06-13-C10 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-13-C10 - Resource: pda17197

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-13-C10 - full reading
    GGAGAGGAAC AACAAGAAAA GTCAAAACGT TGAAGCTTTC TCGAATAACG 
    CCTTCTTTAG TTTACAGTCT CTCGTTGCGG GTCTTATCTT CTTCTTCTTC
    TTCCCTCTTT CGTCTGCTCT ATATTATGAC CAGATTCAAA ATTGCGTAGC
    GTACTAATTC AATATAGAGA GAAGTTAGAT CAAAAGAAGA ACACGAAACT
    CTGACTGTGT CTCTCTTTCT CTTCGTGTGT TTCTATATTT ATATATAAAA
    AGACAAGATC TCTGGTCTGG AATTAGAAGA ATCTTATTTG GGGTTTTTTC
    TTAGGATTAA GCTCTAATGG CAGATCAAGT AAAAGAGCGG ACTTTAGAGG
    AGACCTCTAC GTGGGCAGTT GCTGTTGTTT GCTTTGTCTT ACTCTTTATT
    TCGATTGTCC TCGAACATTC TATTCACAAA ATTGGAACCT GGTTTAAAAA
    GAAGCACAAG CAGGCTCTTT TTGAAGCTCT TGAAAAGGTC AAAGCAGAGC
    TTATGCTGTT GGGATTCATA TCACTACTAC TCACAATTGG ACAAACACCA
    ATCTCAAATA TCTGCATCTC CCAGAAAGTT GCGTCAACAA TGCACCCTTG
    CAGTGCTGCT GAAGAAGCTA AAAAATACGG CAAGAAAGAC GCCGGAAAGA
    AAGATGATGG AGATGGAGAT AAACCCGGTC GAAGACTTCT TCTTGAGTTA
    GCTGAATCTT ATATCCATAG AAGAAGTTTA GCCACCAAAG GCTATGACAA
    ATGTGCAGAG AAGGGGAAAG TGGCTTTTGT ATCTGCTTAT GGAATCCACC
    AGCTGCATAT ATTCATCTTC GTGCTCGCGG TTGTTCATGT TGTTTACTGC
    ATTGTTACTT ATGCTTTCGG AAAGATCAAG ATGAGGACGT GGAAGTCGTG
    GGAGGAAGAG ACAAAGACAA TAGAGTATCA GTATTCCAAC GATCCTGAGA
    GGTTCAGGTT TGCGAGGGAC ACATCTTTTG GGAGAAGACA TCTCAATTTC
    TGGAGCAAGA CGAGAGTCAC ACTATGGATT GTTTGTTTTT TTAGACAGTT
    CTTTGGATCT GTCACCAAAG TTGATTACTT AGCACTAAGA CATGGTTTCA
    TCATGGCGCA TTTTGCTCCC GGTAACGAAT CAAGATTCGA TTTCCGCAAG
    TATATTCAGA GATCATTAGA GAAAGACTTC AAAACCGTTG TTGAAATCAG
    TCCGGTTATC TGGTTTGTCG CTGTGCTATT CCTCTTGACC AATTCATATG
    GATTACGTTC TTACCTCTGG TTACCATTCA TTCCACTAGT CGTAATTCTA
    ATAGTTGGAA CAAAGCTTGA AGTCATAATA ACAAAATTGG GTCTAAGAAT
    CCAAGAGAAA GGTGATGTGG TGAGAGGCGC CCCAGTGGTT CAGCCTGGTG
    ATGACCTCTT CTGGTTTGGC AAGCCACGCT TCATTCTTTT CCTTATTCAC
    TTGGTCCTTT TTACGAATGC ATTTCAACTT GCATTCTTTG CCTGGAGTAC
    GTATGAATTC AATCTCAATA ATTGTTTCCA TGAAAGCACT GCAGATGTGG
    TCATTAGACT TGTAGTTGGA GCTGTTGTGC AGATACTTTG CAGCTATGTG
    ACTCTTCCAC TCTATGCACT TGTTACTCAG ATGGGTAGTA AAATGAAGCC
    AACAGTATTC AACGATAGAG TAGCCACGGC ATTAAAGAAG TGGCATCACA
    CTGCAAAGAA CGAGACGAAA CACGGAAGAC ACTCGGGATC CAACACACCT
    TTCTCTAGCC GTCCAACCAC ACCAACACAT GGCTCATCTC CAATCCATCT
    CCTTCACAAT TTCAATAACC GGAGCGTTGA AAATTACCCA AGTTCTCCTT
    CTCCTAGATA CTCTGGTCAT GGTCATCATG AACACCAATT TTGGGATCCT
    GAGTCTCAAC ACCAAGAAGC TGAAACTTCC ACACATCATT CTCTTGCGCA
    TGAAAGCTCA GAACCTGTTC TTGCATCTGT GGAACTTCCT CCTATAAGGA
    CTAGCAAAAG CTTAAGAGAT TTTTCTTTTA AGAAATGATG ATTCTTGTTT
    GCTATATTTG ATTTCGTACA GTGGGAAATT TGTCATATGA AAATAATTTC
    TTGTACATTA AAAA

    Gene models with high sequence identity

    1. AT1G11310.1 [+] show detail - Seven transmembrane MLO family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    See the detailed result >

    AT1G11310.1

    Model type
    Protein coding
    Short Description
    Seven transmembrane MLO family protein
    Curator Summary
    A member of a large family of seven-transmembrane domain proteins specific to plants, homologs of the barley mildew resistance locus o (MLO) protein. The Arabidopsis genome contains 15 genes encoding MLO proteins, with localization in plasma membrane. Phylogenetic analysis revealed four clades of closely-related AtMLO genes. ATMLO2 belongs to the clade IV, with AtMLO3, AtMLO6 and AtMLO12. The gene is expressed during early seedling growth, in roots, in vascular system of cotyledons and young leaves,and in fruit abscission zone; it was not expressed in anthers and pollen, as shown by GUS activity patterns. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s). mlo resistance in A. thaliana does not involve the signaling molecules ethylene, jasmonic acid or salicylic acid, but requires a syntaxin, glycosyl hydrolase and ABC transporter.
    Computational Description
    MILDEW RESISTANCE LOCUS O 2 (MLO2); FUNCTIONS IN: calmodulin binding; INVOLVED IN: defense response to fungus, incompatible interaction, cell death, response to fungus, negative regulation of defense response, defense response; LOCATED IN: plasma membrane; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Mlo-related protein (InterPro:IPR004326); BEST Arabidopsis thaliana protein match is: Seven transmembrane MLO family protein (TAIR:AT1G61560.1); Has 559 Blast hits to 538 proteins in 60 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 2; Plants - 551; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR