F13119

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13119

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13119

Determination of introduced cDNA(s)

  • RAFL04-19-H23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-19-H23 - Resource: pdz87428

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-19-H23 - 5 prime EST
    GATCTAGAAT CTTCCCTCAA CCTCTAGAAA ACAACTTTCG TATCCTTCAA 
    AAACCCTAAA CCCTAGCTTT TGCACAGACG CAATTTCATC GCATCATTTT
    GCAATTTTCC TTTACTGATC TGTCACCACC TCTTGAATTT CGAAACCATG
    GCTACCGCAG CTCTCCTCCG CTCTATTCGA CGCCGTGAAG TCGTTTCCTC
    TCCTTTCTCA GCTTACAGAT GCCTTTCAAG CAGTGGGAAA GCATCATTGA
    ACAGTTCCTA TCTGGGTCAG AATTTTAGAA GCTTTTCCAG AGCCTTTAGC
    TCGAAGCCTG CTGGAAACGA TGTCATTGGT ATTGATTTGG GTACTACTAA
    TTCATGTGTT GCCGTCATGG AGGGGAAGAA TCCGAAAGTC ATTGAAAATG
    CTGAAGGTGC TCGAACCACT CCATCAGTTG TAGCATTCAA CACGAAAGGA
    GAACTTCTTG TGGGTACACC AGCCAAGCGA CAAGCTGTCA CTAATCCTAC
    AAACACAGTA TCTGGAACGA AACGTTTGAT TGGGAGAAAG TTCGACGATC
    CACAAACTCA GAAAGAAATG AAGATGGTGC CTTACAAGAT TGGGCGTGCA
    CCTAATGGCG ATGCGTGGGT TGAAGCCAAT GGTCAGCAGT ATTCTCCTAG
    TCAGATTGGA GCGTTTATCT TG

    Gene models with high sequence identity

    1. AT5G09590.1 [+] show detail - mitochondrial HSO70 2
      E-value: 0; Score: 99.85

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-19-H23 - 3 prime EST
    GAAAACTTCT TCTCTTTAAC CATCACATTA CATAACAAAG GTTTTTTAGC 
    AGAGAAACAT TAAGTTTAGT TCCCTGACAA AAGCTTATTC CAGAAACATA
    TCCACTGCTC GTTGTTCTAA AAGGCAAAAG TTCCTTGGAC ATAGAACCCA
    CCATGATCTA AACCTAATCT CTTAAAACCC AAGTGGCAAG AATTTACCAA
    ATCTCCTCAC TTTTTCACTT CCTCGTACTC TGCTTCTGGA GCTTGATCAC
    TGCCTCCCTC AGATCCTCCT CCTGGTGCAG AGCCTCCACC AGAACCACCA
    GACATGTGCT CCCCAATCTT AGAAACAGCT TTGTTTGCCG CCTCAATCTT
    GGCCTTGATC TCGTTGAGAT CATCCCCAGA GGAAGCGCTC CTTAGATCTG
    CCACAGCATC TTCAATCTCC TTGGCGATTT CACTTGGGAT CTTCTCTCTG
    TATTCACCAA GACTCTTCTC TATGCTGTAA ATTGTTGTGT CAGCTGTGTT
    CTTGGTGTCG ATCAATTCTT TTCTTTCTTT GTCTTTCTGA GCATGCAACT
    CTGCTTCTCT CACCATCTTC TGGATATCAT CCTCTGAGAG CCCACCAGAG
    GATCGGATTG

    Gene models with high sequence identity

    1. AT5G09590.1 [+] show detail - mitochondrial HSO70 2
      E-value: 0; Score: 100.00

    AT5G09590.1

    Model type
    Protein coding
    Short Description
    mitochondrial HSO70 2
    Curator Summary
    heat shock protein 70 (Hsc70-5); nuclear
    Computational Description
    mitochondrial HSO70 2 (MTHSC70-2); FUNCTIONS IN: ATP binding; INVOLVED IN: protein folding, response to cadmium ion, response to salt stress, response to virus, response to heat; LOCATED IN: mitochondrion, cell wall, plasma membrane, chloroplast, mitochondrial matrix; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Heat shock protein 70, conserved site (InterPro:IPR018181), Chaperone DnaK (InterPro:IPR012725), Heat shock protein Hsp70 (InterPro:IPR001023), Heat shock protein 70 (InterPro:IPR013126); BEST Arabidopsis thaliana protein match is: mitochondrial heat shock protein 70-1 (TAIR:AT4G37910.1); Has 35090 Blast hits to 34955 proteins in 4871 species: Archae - 160; Bacteria - 17405; Metazoa - 3531; Fungi - 1665; Plants - 1246; Viruses - 305; Other Eukaryotes - 10778 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR