F12547

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	decreased width (PATO:0000599) (Cauline leaf::Shape::narrow) epinastic (PATO:0000945) (Cauline leaf::Shape::epinastic)
	fruit (PO:0009001)	abnormal (PATO:0000460) (Silique::Others)
	stem (PO:0009047)	dark green (PATO:0001249) (Stem::Color::dark) abnormal (PATO:0000460) (Stem::Others)
T2
	cauline leaf (PO:0000013)	decreased width (PATO:0000599) (Cauline leaf::Shape::narrow)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) upturned (PATO:0002031) (Rosette leaf::Shape::hyponastic)
	fruit (PO:0009001)	abnormal (PATO:0000460) (Silique::Other)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Shape::zigzag)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12547

Determination of introduced cDNA(s)

RAFL09-74-G13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-74-G13 - Resource: pda08743

full reading [+] show sequence

ATTCTCTCTC TAACTTCTCT ATGTTAAAAT TCCGAAAGCT CGATTCAGAA 
ATCTAGAAGC TTCCTCGAGG GCCCTCGCGT AGAAAGTCGA ACTCAAATTC 
CCTGGAGACA AAGAAGAGGA AGATGAGCTC ATGGAACTAC GTTGTTACGG 
CTCACAAACC GACCAGTGTC ACTCACTCGT GCGTTGGCAA CTTCACAAGT 
CCTCAAGAGC TCAATCTCAT TGTCGCGAAA TGTACTCGAA TCGAGATCCA 
TTTGCTTACC CCACAAGGAC TTCAGCCTAT GTTGGACGTT CCTATATATG 
GAAGAATCGC TACTCTGGAG TTGTTCCGAC CCCATGGCGA AGCGCAAGAC 
TTTTTGTTCA TTGCAACTGA GAGATACAAA TTCTGTGTTC TTCAATGGGA 
TCCTGAGTCC TCTGAGCTTA TTACAAGAGC AATGGGGGAT GTTTCTGATC 
GTATAGGCCG GCCAACAGAC AATGGTCAGA TTGGTATAAT TGATCCAGAT 
TGTAGATTAA TTGGACTCCA TCTGTATGAC GGCTTGTTCA AGGTTATTCC 
ATTTGATAAT AAGGGACAGC TCAAGGAAGC TTTTAATATC AGGCTTGAGG 
AATTGCAGGT TCTGGATATC AAGTTTCTGT TTGGATGTGC AAAGCCCACT 
ATTGCAGTAC TATATCAGGA CAACAAAGAT GCTCGTCATG TCAAAACATA 
TGAAGTCTCT CTTAAAGATA AGGATTTTGT CGAGGGTCCA TGGTCTCAGA 
ACAGTCTCGA CAATGGTGCT GACTTGCTGA TTCCTGTACC ACCACCGCTC 
TGTGGTGTCC TTATTATTGG TGAAGAAACA ATTGTCTATT GCAGTGCTAG 
TGCATTCAAA GCAATACCAA TAAGACCTTC CATCACTAAA GCATATGGAA 
GAGTTGATGT TGATGGCTCT AGATATCTTC TTGGTGACCA TGCTGGAATG 
ATTCATCTGC TTGTTATAAC GCACGAGAAA GAAAAGGTCA CTGGCCTAAA 
AATTGAGCTT TTGGGTGAAA CATCTATTGC ATCGACTATA TCGTACCTTG 
ACAATGCCGT TGTCTTTGTC GGCTCAAGCT ATGGTGATTC ACAGCTAGTA 
AAGCTGAATC TACACCCTGA TGCAAAAGGC TCATATGTAG AAGTCTTGGA 
AAGGTATATC AACTTGGGGC CTATTGTCGA CTTTTGTGTA GTTGACCTCG 
AGAGACAGGG GCAAGGTCAG GTTGTAACTT GTTCTGGAGC ATTTAAGGAT 
GGTTCCCTCC GCGTAGTTCG CAATGGAATA GGAATCAATG AACAGGCGTC 
TGTGGAACTT CAAGGTATCA AAGGAATGTG GTCGTTGAAA TCTTCAATTG 
ATGAAGCCTT CGATACATTT CTTGTTGTTA GCTTTATCAG TGAAACTCGC 
ATATTAGCCA TGAATCTTGA GGATGAACTG GAAGAAACAG AGATTGAGGG 
CTTCTTGTCT CAAGTGCAGA CGTTATTTTG CCATGATGCT GTGTACAATC 
AACTTGTACA GGTTACCTCA AATTCTGTTA GACTAGTCAG TTCTACAACT 
AGAGAATTGC GGGACGAGTG GCATGCCCCA GCTGGATTCA CTGTTAATGT 
TGCAACTGCA AATGCCAGCC AGGTTCTTTT GGCGACTGGA GGTGGGCATT 
TGGTTTATCT AGAAATTGGA GATGGGAAAT TGACGGAAGT GCAACATGCC 
CTTTTGGAGT ACGAGTTTTC CTGTCTTGAT ATAAACCCTA TCGGTGACAA 
TCCTAATTAC AGTCAGCTAG CTGCGGTTGG GATGTGGACA GATATAAGTG 
TGAGGATCTT TTCGCTGCCA GAATTGACTC TTATTACAAA AGAGCAACTT 
GGAGGGGAAA TAATTCCCCG ATCTGTTCTT CTTTGTGCAT TCGAAGGGAT 
ATCTTACTTG CTATGTGCTC TTGGAGATGG CCATCTTTTG AACTTCCAGA 
TGGATACAAC TACCGGACAA TTAAAAAAAA AAAAAAAA

Gene models with high sequence identity

AT4G05420.1 [+] show detail - damaged DNA binding protein 1A
E-value: 0; Score: 99.95

InterPro Scan Digest

Program	Description	E-value
Coil	coiled-coil	0
HMMPanther	DNA REPAIR PROTEIN XP-E	0
HMMPanther	DNA REPAIR/RNA PROCESSING CPSF FAMILY	0

See the detailed result >

AT4G05420.1

Model type: Protein coding
Short Description: damaged DNA binding protein 1A
Curator Summary: Structurally similar to damaged DNA binding proteins.DDB1a is part of a 350 KDa nuclear localized DET1 protein complex. This complex may physically interact with histone tails and while bound to chromatin- repress transcription of genes involved in photomorphogenesis.
Computational Description: damaged DNA binding protein 1A (DDB1A); FUNCTIONS IN: protein binding, DNA binding; INVOLVED IN: negative regulation of transcription, negative regulation of photomorphogenesis; LOCATED IN: nucleus, CUL4 RING ubiquitin ligase complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat-like-containing domain (InterPro:IPR011046), Cleavage/polyadenylation specificity factor, A subunit, C-terminal (InterPro:IPR004871); BEST Arabidopsis thaliana protein match is: damaged DNA binding protein 1B (TAIR:AT4G21100.1); Has 1490 Blast hits to 1182 proteins in 227 species: Archae - 0; Bacteria - 0; Metazoa - 604; Fungi - 374; Plants - 231; Viruses - 0; Other Eukaryotes - 281 (source: NCBI BLink).
Link: InterPro Scan - TAIR