F12226

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small) dark green (PATO:0001249) (Cauline leaf::Color::dark)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) increased size (PATO:0000586) (Rosette leaf::Shape::large)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	root (PO:0009005)	present in greater numbers in organism (PATO:0000470) (Root::Number::many)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick) increased thickness (PATO:0000591) (Stem::Shape::thick) abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Others)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)
T2
	cauline leaf (PO:0000013)	increased size (PATO:0000586) (Cauline leaf::Shape::large) dark green (PATO:0001249) (Cauline leaf::Color::dark) abnormal (PATO:0000460) (Cauline leaf::Others)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) increased size (PATO:0000586) (Rosette leaf::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12226

Determination of introduced cDNA(s)

RAFL09-13-B09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-13-B09 - Resource: pda01703

full reading [+] show sequence

GTCTTCCGTT GCGTGAGATA TTATTGAACG GTGTTTGTTA TTCGGAGAAG 
AAGAAGAAGA AGAACTGAAG AAGCATATTC GACGGTCACC ACCAAACCTC 
TTCCGTCGGA TTTTCACTCT CCGTTTATCG AACATCTGAG ATCTCTCTCC 
ACTTCCGTCA CCGAAGATGC ATTCCAGTCA TCTTCTTCTC GAGGAGCCGA 
TCAGGATGGC TTCAATCCTC GAGCCTTCCA AATCTAGTTT CTTCCCGGCA 
TTGACTAAGA TCGTCGGAAC TCTAGGTCCT AAATCCCGAT CCGTCGAGGC 
TCTCTCCGGC TGTCTCAAAG CCGGCATGTC TGTGGCTCGA TTTGATTTCT 
CGTGGGGAGA TGCTGATTAT CACCAGGAGA CACTTGATAA TTTGAAAGTT 
GCTGTGAGGA GCACTAAGAA GCTTTGTGCT GTTATGCTTG ATACTGTTGG 
ACCTGAGCTA CAAGTTATTA ACAAATCTGA GAAAGCTATT ACTTTGAAAG 
CTGATGGCCT TGTAACTTTG ACACCGAATC AAGATCAAGA AGCTTCTTCT 
GAAGTTCTTC CCATTAATTT CAATGGACTT GCCAAGGCAG TGAAGAAAGG 
AGACACCATC TTCGTTGGGC AATACCTATT CACTGGTAGT GAAACAACTT 
CAGTTTGGCT CGAGGTTGAT GAAGTTAAAG GAGATGATGT CATTTGCCTT 
TCAAGGAATG CTGCTACTCT GGCTGGTTCT TTGTTCACTT TACACTCCTC 
TCAAGTTCAC ATTGATCTCC CAACGCTTAC AGAGAAGGAT AAGGAGGTTA 
TAAGCACATG GGGAGTTCAA AATAAAATCG ATTTTCTCTC ATTGTCTTAT 
TGTCGTCATG CTGAGGATGT TCGCCAGACC CGTGAAATGC TTAAAAAGTT 
GGGCGACCTC TCTCAAACAC AAATATTTGC TAAGATTGAG AACGTAGAGG 
GACTAACCCA CTTCGATGAG ATTCTACAAG AAGCTGATGG CATTATTCTT 
TCTCGTGGGA ATTTGGGTAT AGATTTACCC CCGGAAAAGG TGTTTTTGTT 
TCAAAAGGCT GCTCTTTACA AGTGCAACAT GGCTGGAAAG CCAGCTGTTC 
TTACCCGTGT TGTTGACAGT ATGACTGACA ACTTGCGACC AACTCGTGCT 
GAGGCCACGG ATGTTGCTAA TGCTGTTTTA GATGGAAGTG ATGCAATTCT 
TCTTGGTGCT GAGACCCTTC GTGGATTGTA CCCTGTTGAG ACAATATCAA 
CTGTTGGTAG GATCTGTGCT GAGGCAGAAA AGGTTTTCAA TCAAGATTTG 
TACTTTAAGA AGACTGTCAA GTATGTTGGA GAACCAATGA CTCACTTGGA 
ATCGATTGCT TCTTCAGCTG TACGGGCAGC CATTAAGGTT AAGGCATCCG 
TAATTATATG CTTCACCTCT TCTGGAAGAG CAGCCAGATT GATTGCCAAA 
TACAGGCCAA CGATGCCGGT TATTTCTGTT GTCATTCCCC GGGTTAAGAC 
AAATCAACTG AAATGGAGCT TTAGTGGAGC TTTTGAGGCG AGGCAGTCAC 
TTATTGTCAG AGGCCTCTTC CCTATGCTTG CTGACCCTCG TCACCCTGCG 
GAATCTACAA GTGCGACAAA CGAGTCAGTC CTGAAGGTTG CTCTAGACCA 
TGGGAAGCAT GCTGGAGTAA TCAAGTCGCA TGACAGAGTA GTGGTGTGTC 
AGAAAGTTGG TGATGCATCT GTGGTTAAGA TCATTGAGCT TGAGGATTAA 
CTTTTGCCTC GCAAAAACAT TATATAATTG GCTGCATTTT GGGTTGCCTT 
GAATTGATGA TAACTCCCAA TGCCGGAGTG AAAATATTAT CTCCGGGACT 
TCTGGTTTCT TTTTGTATAC TTAGGAGATT TGATGTCTCT AAGGTTTCAG 
CTATATTTGA TTACCTTCAA ATGTACGCAT ATGATAAAAG TATACAATGC 
CTAGCAATTT TATAATAAGC TGTTACATTC CCTT

Gene models with high sequence identity

AT3G52990.1 [+] show detail - Pyruvate kinase family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
superfamily	Pyruvate/Phosphoenolpyruvate kinase, catalytic core	6.6e-71
superfamily	Pyruvate kinase, C-terminal-like	4e-27
superfamily	Pyruvate kinase, beta-barrel-like	1.4e-08
Gene3D	Pyruvate/Phosphoenolpyruvate kinase, catalytic core	1.4e-66
Gene3D	Pyruvate kinase, alpha/beta	6.3e-18
HMMPfam	Pyruvate kinase, barrel	2.9e-93
HMMPfam	Pyruvate kinase, alpha/beta	8.2e-23
HMMPanther	Pyruvate kinase	5.2e-206
HMMTigr	Pyruvate kinase	2.5e-99
FPrintScan	Pyruvate kinase, barrel	1.2e-43
FPrintScan	Pyruvate kinase, barrel	1.2e-43
FPrintScan	Pyruvate kinase, barrel	1.2e-43
FPrintScan	Pyruvate kinase, barrel	1.2e-43
FPrintScan	Pyruvate kinase, barrel	1.2e-43
FPrintScan	Pyruvate kinase, barrel	1.2e-43

See the detailed result >

AT3G52990.1

Model type: Protein coding
Short Description: Pyruvate kinase family protein
Computational Description: Pyruvate kinase family protein; FUNCTIONS IN: pyruvate kinase activity, potassium ion binding, magnesium ion binding, catalytic activity; INVOLVED IN: response to cadmium ion, glycolysis; LOCATED IN: membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Pyruvate/Phosphoenolpyruvate kinase, catalytic core (InterPro:IPR015813), Pyruvate kinase, alpha/beta (InterPro:IPR015794), Pyruvate kinase, beta-barrel-like (InterPro:IPR011037), Pyruvate kinase (InterPro:IPR001697), Pyruvate kinase, C-terminal-like (InterPro:IPR015795), Pyruvate kinase, barrel (InterPro:IPR015793); BEST Arabidopsis thaliana protein match is: Pyruvate kinase family protein (TAIR:AT2G36580.1); Has 9836 Blast hits to 9793 proteins in 2683 species: Archae - 167; Bacteria - 5933; Metazoa - 534; Fungi - 219; Plants - 549; Viruses - 0; Other Eukaryotes - 2434 (source: NCBI BLink).
Link: InterPro Scan - TAIR