F09720

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F09720

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
stem (PO:0009047)
  • present in greater numbers in organism (PATO:0000470)
    (Stem::Number::many)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09720

Determination of introduced cDNA(s)

  • RAFL05-20-P15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-20-P15 - Resource: pda01337

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-20-P15 - full reading 
    ACAACCGATT TTCTCCATTT TCTTCAAAAC GATTCGGGTC AAGGTTATTG 
    ACTTTCTCAT TCAGTGAAGC TTGAATCATG GCCTCGAGAA TTGCTAAAGA 
    TGTGACTGAA TTGATTGGGA ACACTCCATT GGTGTTGAAC AATGTTGCTG 
    AAGGATGTGT TGGTCGTGTT GCTGCTAAGC TTGAGATGAT GGAACCGTGC 
    TCTAGCGTCA AAGACAGGAT TGGTTTTAGC ATGATTTCTG ATGCAGAAAA 
    GAAGGGTCTT ATCAAACCAG GAGAGAGTGT GCTGATTGAG CCAACAAGTG 
    GAAACACTGG AGTTGGGTTA GCATTCACGG CAGCTGCCAA AGGCTACAAG 
    CTTATTATTA CAATGCCAGC TTCTATGAGT ACTGAGAGAA GAATCATTCT 
    CTTAGCTTTT GGAGTTGAGT TGGTTTTAAC TGACCCAGCT AAGGGCATGA 
    AAGGAGCTAT CGCAAAGGCG GAAGAGATTT TGGCGAAAAC ACCCAATGGT 
    TACATGCTTC AGCAGTTTGA GAACCCTGCC AACCCTAAGA TCCACTATGA 
    GACTACGGGA CCTGAGATAT GGAAAGGCAC TGGTGGCAAA ATCGATGGCT 
    TTGTTTCTGG GATTGGTACT GGTGGTACCA TTACAGGTGC TGGGAAGTAT 
    CTTAAAGAAC AGAACGCAAA CGTCAAGCTG TATGGAGTGG AGCCAGTTGA 
    AAGTGCTATT CTATCCGGTG GGAAGCCAGG TCCTCACAAG ATTCAAGGGA 
    TAGGAGCTGG TTTTATACCA AGTGTATTGA ATGTTGATCT TATTGACGAA 
    GTTGTTCAGG TTTCAAGTGA TGAATCCATT GACATGGCAA GGCAGCTTGC 
    TCTTAAAGAA GGTCTTCTTG TGGGAATATC ATCCGGTGCA GCAGCTGCTG 
    CAGCAATTAA ACTTGCACAG AGGCCAGAAA ACGCTGGGAA GCTATTTGTG 
    GCGATATTCC CGAGTTTCGG GGAGAGGTAT CTATCAACGG TACTTTTCGA 
    TGCGACAAGG AAAGAAGCGG AAGCCATGAC CTTCGAGGCT TGAACATTCT 
    CCATTTCTTC TTAAGAGACG CCAAAATAAA AGAGATGTTC AGTTTCTCCT 
    ATAGAGACTC TTCATCTTTA GTTACATTGC GTCTTTGCTT CCATCTGTAT 
    CTTCTCTTGT GTGCCAATAA AAGTCAAACT AGATTTTCTC TGTTTCTTTT 
    GTGAACCACT TGCTTGTTAA TGAAGTTTAA ATTTGCCAAA AAAAAAAAAA 
    AAAA

    Gene models with high sequence identity

    1. AT4G14880.1 [+] show detail - O-acetylserine (thiol) lyase (OAS-TL) isoform A1
      E-value: 0; Score: 99.76

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Pyridoxal phosphate-dependent enzyme, beta subunit 1.8e-91
    HMMPanther CYSTEINE SYNTHASE 1.3e-145
    HMMPanther SER/THR DEHYDRATASE, TRP SYNTHASE 1.3e-145
    PatternScan Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site 0
    Gene3D no description 2.6e-68
    HMMTigr Cysteine synthase K/M 2.5e-214
    HMMTigr Cysteine synthase A 1.1e-228
    HMMPfam Pyridoxal phosphate-dependent enzyme, beta subunit 1.4e-54

    See the detailed result >

    AT4G14880.1

    Model type
    Protein coding
    Short Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1
    Curator Summary
    Encodes a cytosolic isoform of cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis and for the fixation of inorganic sulfide. It catalyzes the formation of cysteine from O-acetylserine and inorganic sulfide. Gene expression is predominant in the root cortex and the xylem parenchyma. Gene expression is induced in leave, stems and roots by high salt and heavy metal stresses, mediated by ABA. Lines carrying semi-dominant mutations exhibit early senescence.
    Computational Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1 (OASA1); FUNCTIONS IN: protein binding, cysteine synthase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site (InterPro:IPR001216), Cysteine synthase A (InterPro:IPR005859), Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Cysteine synthase K/M (InterPro:IPR005856); BEST Arabidopsis thaliana protein match is: O-acetylserine (thiol) lyase (OAS-TL) isoform A2 (TAIR:AT3G22460.1); Has 20496 Blast hits to 20479 proteins in 2663 species: Archae - 403; Bacteria - 14284; Metazoa - 350; Fungi - 659; Plants - 549; Viruses - 2; Other Eukaryotes - 4249 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR