F05922

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F05922

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in greater numbers in organism (PATO:0000470)
    (Stem::Number::many)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F05922

Determination of introduced cDNA(s)

  • RAFL05-21-C12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-21-C12 - Resource: pda01322

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-21-C12 - full reading 
    AACACACGCC TCTAATCTCC GCGCACACAC ACACACCCTC ACGAAGAAGA 
    AGAAGACGAA GAAGAATCAA TGGCTTCTGT TATCTCTTCC TCTCCTTTTC 
    TATGCAAATC ATCCTCCAAG AGTGATTTGG GGATTTCTTC GTTTCCTAAA 
    TCTTCTCAGA TTTCGATTCA TCGATGTCAG AAGAAATCGA TCTCACGGAA 
    GATTGTTTCC GTTATGGCTC CTCAAAAGGA TCGGTCTCCT GGAACTACAG 
    GATCGGTGAA AACTGGGATG ACAATGACGG AGAAGATTCT AGCTAGAGCT 
    TCGGAGAAGT CATTAGTGGT TCCTGGTGAT AACATTTGGG TTAATGTTGA 
    TGTTCTTATG ACTCATGATG TTTGTGGTCC TGGTGCTTTT GGTATCTTCA 
    AGAGAGAGTT TGGTGAAAAA GCCAAGGTTT GGGATCCGGA GAAGATTGTT 
    GTGATTCCGG ATCATTATAT TTTCACTGCT GATAAGCGTG CAAACCGGAA 
    TGTTGACATC ATGAGGGAGC ATTGCAGGGA ACAGAATATT AAGTATTTCT 
    ATGATATCAC TGACCTTGGA AATTTTAAGG CTAATCCTGA CTACAAAGGT 
    GTTTGCCATG TTGCACTTGC ACAAGAAGGT CATTGCAGGC CAGGAGAGGT 
    TTTGTTAGGA ACAGACTCAC ACACTTGTAC TGCTGGAGCA TTTGGTCAAT 
    TTGCTACAGG GATTGGAAAC ACTGATGCAG GTTTTGTGTT AGGCACTGGA 
    AAAATCCTCC TTAAGGTTCC ACCAACGATG AGGTTTATCT TGGATGGTGA 
    GATGCCTAGT TATTTGCAAG CAAAGGATCT GATTTTACAA ATTATTGGTG 
    AAATATCTGT TGCTGGTGCA ACTTACAAGA CGATGGAGTT CAGTGGTACA 
    ACTATCGAAA GTCTGAGTAT GGAAGAAAGA ATGACATTGT GCAACATGGT 
    TGTGGAAGCT GGGGGAAAGA ATGGTGTCAT CCCTCCTGAT GCGACGACAT 
    TAAATTATGT TGAGAATAGG ACATCTGTAC CATTTGAGCC AGTATATAGT 
    GATGGAAATG CAAGCTTTGT CGCAGATTAT AGATTTGACG TGTCAAAGCT 
    GGAGCCTGTG GTGGCTAAGC CTCATTCTCC TGACAACCGA GCTCTAGCTA 
    GAGAATGCAA AGACGTGAAA ATTGACAGAG TATACATCGG TTCTTGTACT 
    GGTGGGAAGA CAGAGGATTT TATGGCTGCA GCTAAACTTT TCCACGCAGC 
    AGGAAGAAAG GTCAAAGTTC CAACTTTCCT TGTCCCGGCT ACTCAGAAGG 
    TGTGGATGGA TGTGTATGCT CTCCCGGTAC CTGGAGCAGG TGGAAAGACA 
    TGTGCGCAGA TCTTTGAAGA AGCTGGCTGT GACACACCAG CCAGCCCTAG 
    CTGTGGTGCT TGCCTTGGTG GCCCAGCAGA CACCTACGCT CGCTTGAATG 
    AACCTCAAGT GTGTGTCTCA ACGACAAACA GGAACTTCCC CGGTCGGATG 
    GGACACAAAG AAGGGCAAAT TTACTTAGCG TCTCCTTACA CTGCTGCAGC 
    CTCGGCTTTA ACCGGCCGTG TCGCTGACCC AAGGGAGTTC TTGCAGTAGG 
    CTAATGTAAT GGCTTCGTGT GAAAAATCAT ATGCTATGCA TCGACGAAAT 
    ATTGCTAAAT GATGGGGCAT TGTGAACTAT ATGTATGTAT TTTCCTTATT 
    TCGCTGTTGG ACTTGATAGT TTCAAGCACT ATGTCAAACT TTGATGTTTT 
    GAGTTTGAAA TAATAAAAGA GAGAAAAAGA GTGAGTTAGA TTAAAAAAAA 
    AAAAAAAA

    Gene models with high sequence identity

    1. AT4G13430.1 [+] show detail - isopropyl malate isomerase large subunit 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    HMMPanther Homoaconitase/3-isopropylmalate dehydratase, small/large subunit 5.9e-194
    HMMPanther Aconitase-like core 5.9e-194
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 1.7e-28
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 3.5e-28
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 2.9e-39
    HMMTigr Homoaconitase/3-isopropylmalate dehydratase, large subunit, subgroup 2e-143
    superfamily Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 2.3e-124
    HMMPfam Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 7.9e-53
    HMMPfam Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 2.2e-19

    See the detailed result >

    AT4G13430.1

    Model type
    Protein coding
    Short Description
    isopropyl malate isomerase large subunit 1
    Curator Summary
    Encodes methylthioalkylmalate isomerase.
    Computational Description
    isopropyl malate isomerase large subunit 1 (IIL1); FUNCTIONS IN: lyase activity, intramolecular transferase activity, transferring hydroxy groups; INVOLVED IN: glucosinolate biosynthetic process, response to cadmium ion, metabolic process; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha (InterPro:IPR001030), Homoaconitase/3-isopropylmalate dehydratase, small/large subunit (InterPro:IPR015936), Homoaconitase/3-isopropylmalate dehydratase, large subunit, subgroup (InterPro:IPR006251), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 (InterPro:IPR015932), Aconitase-like core (InterPro:IPR015937), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (InterPro:IPR015931); BEST Arabidopsis thaliana protein match is: aconitase 2 (TAIR:AT4G26970.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR