About RAFL cDNAs
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
We have determined full-length sequences of RAFL cDNA clones in collaboration with the Arabidopsis SSP group of the USA, which comprises the Salk Institute (PI: Dr. Joseph R. Ecker), the Stanford Genome Technology Center (PI: Dr. Ronald W. Davis), and the Plant Gene Expression Center (PI: Dr. Athanasios Theologis). After determination of the full-length sequences of the RAFL cDNA clones, the RAFL cDNA clones are available from the RIKEN Bioresource Center (BRC) (http://www.brc.riken.jp/lab/epd/Eng/species/arabidopsis.shtml).
-RAFL cDNAs Keyword Search
-Blast for cDNA 3' flanking sequences
-Blast for cDNA full-length sequences